TET1 Double Nickase Plasmid (h): sc-400845-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TET1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TET1 Double Nickase Plasmid (h) and TET1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TET1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TET1 Antibody (4F4): sc-293186
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TET1 Double Nickase Plasmid (h) | sc-400845-NIC | 20 µg | $410.00 | |||
TET1 Double Nickase Plasmid (h2) | sc-400845-NIC-2 | 20 µg | $410.00 |
TET1 encodes a methylcytosine dioxygenase that catalyzes stepwise oxidation of 5-methylcytosine to 5-hydroxymethylcytosine and further oxidized derivatives, supporting active and passive DNA demethylation. Through modulation of CpG methylation landscapes, TET1 contributes to transcriptional regulation, enhancer activity, and chromatin state transitions that shape cell fate decisions and developmental programs. TET1 integrates with epigenetic regulatory networks involving DNA methyltransferases, base excision repair components, and chromatin modifiers to maintain genome-wide epigenomic plasticity. Altered TET1 activity or 5hmC distribution is associated with dysregulated gene expression observed in cancer biology, neurodevelopmental processes, and immune cell differentiation, making it a useful node for mechanistic studies of epigenetic instability.
TET1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TET1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TET1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TET1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TET1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.