Anti-Testican-2 Antibody (B-5) recommended for detection of Testican-2 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Testican-2 Antibody (B-5): sc-515691

Anti-Testican-2 Antibody (B-5) is rated 4.0 out of 5 by 1.
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Datasheets

    • Anti-Testican-2 Antibody (B-5) is a mouse monoclonal IgG2b (kappa light chain) Testican-2 antibody provided at 200 µg/ml
    • raised against amino acids 293-340 mapping near the C-terminus of Testican-2 of human origin
    • Anti-Testican-2 Antibody (B-5) is recommended for detection of Testican-2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Testican-2 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Testican-2 (B-5): sc-515691.
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How much of the Testican-2 antibody do you recommend using for ELISA? Thank you!

Asked by: AAAJ
Thank you for your question. We don't have a recommended dilution for ELISA assay. It's best to optimize your antibody amount by titration. Please contact Technical Service for further assistance.
Answered by: Technical Service 3
Date published: 2020-11-12

What's the immunogen of the Testican-2 antibody

Asked by: Don Wen
Thank you for your question. Testican-2 Antibody (B-5): sc-515691 has been raised against amino acids 293-340 mapping near the C-terminus of Testican-2 of human origin.
Answered by: Tech Support Europe
Date published: 2020-08-10

How much sample do you recommend loading on the gel for Western blot detection using Testican-2 (B-5): sc-515691?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from good antibody I used this antibody for WB on human blood samples, it works well.
Date published: 2017-10-23
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