TESPA1 CRISPR/Cas9 KO Plasmid (h): sc-406563
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TESPA1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TESPA1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TESPA1 CRISPR/Cas9 KO Plasmid (h) | sc-406563 | 20 µg | $397.00 |
Overview
TESPA1 (thymocyte-expressed, positive selection-associated 1) encodes an adaptor protein enriched in lymphoid cells that coordinates signal transduction downstream of antigen receptors. In T cells, TESPA1 contributes to proximal TCR signaling and calcium mobilization by linking receptor engagement to PLCγ-dependent pathways and endoplasmic reticulum Ca²⁺ release, thereby shaping activation programs and thymocyte selection. It is also implicated in B cell receptor signaling and broader immune-cell communication networks that influence cytokine responses and differentiation. Altered TESPA1 activity has been associated with immune dysregulation phenotypes, including susceptibility to autoimmune and inflammatory disease mechanisms, making it relevant for studying signaling thresholds and lymphocyte function.
TESPA1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TESPA1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TESPA1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TESPA1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TESPA1 protein expression.
This CRISPR knockout system enables efficient generation of TESPA1-deficient cell models for investigation of TESPA1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TESPA1 exon(s) critical for TESPA1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TESPA1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TESPA1 CRISPR/Cas9 KO Plasmid (h) and TESPA1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TESPA1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TESPA1 HDR Plasmid (h) and TESPA1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TESPA1 homology arms to support homology-directed repair at defined TESPA1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.