Tesmin CRISPR/Cas9 KO Plasmid (h): sc-410474
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tesmin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Tesmin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tesmin CRISPR/Cas9 KO Plasmid (h) | sc-410474 | 20 µg | $397.00 |
Overview
TESMIN (also known as MTL5) encodes Tesmin, a metallothionein-like protein with prominent roles in metal ion homeostasis and cellular responses to zinc and other divalent cations. Tesmin has been linked to regulation of transcriptional programs and cell-cycle–associated processes, with reported nuclear localization dynamics that suggest context-dependent control of gene expression. Altered TESMIN/MTL5 expression has been observed across multiple cancer datasets and correlated with proliferative phenotypes, supporting its relevance to tumor biology and stress-adaptation pathways. As a metal-binding protein connected to redox balance and cellular differentiation states, Tesmin is of interest for dissecting how metal signaling interfaces with growth control and genomic stability.
Tesmin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TESMIN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TESMIN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TESMIN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Tesmin protein expression.
This CRISPR knockout system enables efficient generation of TESMIN-deficient cell models for investigation of Tesmin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TESMIN exon(s) critical for Tesmin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TESMIN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Tesmin CRISPR/Cas9 KO Plasmid (h) and Tesmin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TESMIN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Tesmin HDR Plasmid (h) and Tesmin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TESMIN homology arms to support homology-directed repair at defined TESMIN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.