Anti-TEM8 Antibody (4H261) is a mouse monoclonal IgG1 (kappa light chain) TEM8 antibody provided at 100 µg/ml
raised against amino acids 481-497 of TEM8 of human origin
recommended for detection of TEM8 of mouse, rat and human origin by WB, IP, IF and IHC(P)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TEM8 (4H261): sc-73136.
m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TEM8 Antibody (4H261) for WB and IHC(P) applications. These reagents are now offered in bundles with TEM8 Antibody (4H261) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
TEM8 Antibody (4H261) is a high quality monoclonal TEM8 antibody (also designated TEM8 antibody) suitable for the detection of the TEM8 protein of mouse, rat and human origin. TEM8 Antibody (4H261) is available as the non-conjugated anti-TEM8 antibody. The tripartite toxin secreted by Bacillus anthraci is the causative agent of anthrax evading the immune system and killing the host during a systemic infection. Two components of the toxin, odemema factor (OF) and lethal factor (LF) enzymatically modify substrates within the cytosol of mammalian cells. The third component, protective antigen (PA), binds to a cellular receptor, designated ATR (anthrax toxin receptor), which mediates the delivery of the enzymatic components to the cytosol. TEM8 (tumor endothelial marker 8) is one of the tumor specific endothelial markers (TEMs) whose N-terminus encodes ATR. TEM8 is highly expressed in tumor endothelial cells but not in normal endothelial cells. TEMs have elevated expression during tumor angiogenesis. Four TEM genes, TEM1, TEM5, TEM7 and TEM8, encode the TEM proteins, which contain putative transmembrane domains. ATR is a type I membrane protein with an extracellular von Willebrand factor A domain that binds directly to PA. The first 364 amino acids of ATR protein are identical to those of TEM8. However, the C-terminal ends of the ATR and TEM8 proteins are different, presumably due to alternative splicing. A soluble version of von Willebrand factor A domain seems to protect cells from the toxin action.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.