TEM1 Antibody (G-9): sc-377221

TEM1 Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects TEM1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TEM1 (G-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tumor endothelial marker 1 (TEM1), also known as endosialin, is a heavily glycosylated, type I transmembrane C-type lectin-like receptor belonging to the Ras superfamily, primarily expressed in the vascular endothelium and fibroblast-like cells during organ development, with expression significantly diminishing in adulthood. The structure of TEM1 comprises three epidermal growth factor-like domains, a C-type lectin domain, and a Sushi domain, which collectively contribute to functional roles in tumor biology. TEM1 is highly upregulated in the endothelium of tumors, where TEM1 plays a critical role in tumor growth and progression, making TEM1 a significant target for anti-angiogenic therapies. TEM1 may facilitate the concentration of liposomes on the surface of target cells, enhancing their fusion with the cell membrane, thereby influencing therapeutic delivery mechanisms.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TEM1 Antibody (G-9) References:

1. Molecular cloning and characterization of endosialin, a C-type lectin-like cell surface receptor of tumor endothelium.  |  Christian, S., et al. 2001. J Biol Chem. 276: 7408-14. PMID: 11084048
2. Molecular characterization of the mouse Tem1/endosialin gene regulated by cell density in vitro and expressed in normal tissues in vivo.  |  Opavsky, R., et al. 2001. J Biol Chem. 276: 38795-807. PMID: 11489895
3. Cell surface tumor endothelial markers are conserved in mice and humans.  |  Carson-Walter, EB., et al. 2001. Cancer Res. 61: 6649-55. PMID: 11559528
4. Human endosialin (tumor endothelial marker 1) is abundantly expressed in highly malignant and invasive brain tumors.  |  Brady, J., et al. 2004. J Neuropathol Exp Neurol. 63: 1274-83. PMID: 15624764
5. Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium.  |  MacFadyen, JR., et al. 2005. FEBS Lett. 579: 2569-75. PMID: 15862292
6. Tumor endothelial marker 1 (Tem1) functions in the growth and progression of abdominal tumors.  |  Nanda, A., et al. 2006. Proc Natl Acad Sci U S A. 103: 3351-6. PMID: 16492758
7. Mouse endosialin, a C-type lectin-like cell surface receptor: expression during embryonic development and induction in experimental cancer neoangiogenesis.  |  Rupp, C., et al. 2006. Cancer Immun. 6: 10. PMID: 16875435
8. Endosialin is expressed on stromal fibroblasts and CNS pericytes in mouse embryos and is downregulated during development.  |  MacFadyen, J., et al. 2007. Gene Expr Patterns. 7: 363-9. PMID: 16965941

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TEM1 Antibody (G-9) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-TEM1 Antibody (G-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TEM1 Antibody (G-9) Specifications

Alternate Product Name: TEM1

Product Clone Name: G-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 105-167

Epitope Target: TEM1

Epitope Species: human

Product Reactivity/Detection: TEM1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for TEM1 Antibody (G-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin