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TCR V β 8.1-2 Antibody (KJ16) - Western Blotting - Image 38339
Western blot analysis of TCR V β 8.1-2 expression in T cell hybridoma, SKK-45.10, immunoprecipitated with TCR V β 8.1-2 Antibody (KJ16): sc-59387 (A) and T cell hybridoma, DO-11.10, immunoprecipitated with TCR V β 8.1-2 Antibody (KJ16): sc-59387 (B). Kindly provided by National Jewish Health.
TCR V β 8.1-2 Antibody (KJ16) - Western Blotting - Image 38339
Western blot analysis of TCR V β 8.1-2 expression in T cell hybridoma, SKK-45.10, immunoprecipitated with TCR V β 8.1-2 Antibody (KJ16): sc-59387 (A) and T cell hybridoma, DO-11.10, immunoprecipitated with TCR V β 8.1-2 Antibody (KJ16): sc-59387 (B). Kindly provided by National Jewish Health.
TCR V β 8.1-2 Antibody (KJ16) - Western Blotting - Image 38338
Western blot analysis of TCR V β 8.1-2 expression in D0-11.10 immunoprecipitated with TCR V β 8.1-2 Antibody (KJ16): sc-59387. Note presence of non-denatured and denatured forms. Kindly provided by National Jewish Health.
Western blot analysis of TCR V β 8.1-2 expression in T cell hybridoma, SKK-45.10, immunoprecipitated with TCR V β 8.1-2 Antibody (KJ16): sc-59387 (A) and T cell hybridoma, DO-11.10, immunoprecipitated with TCR V β 8.1-2 Antibody (KJ16): sc-59387 (B). Kindly provided by National Jewish Health.

TCR V β 8.1-2 Antibody (KJ16): sc-59387

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Datasheets
  • TCR V β 8.1-2 Antibody (KJ16) is a rat monoclonal IgG TCR V β 8.1-2 antibody provided at 200 µg/ml
  • raised against TCR isolated from NP-40 lysates of DO-11.10 mouse cells
  • recommended for detection of TCR V β 8.1 and TCR V β 8.2 of the murine T cell receptor of mouse origin by WB, IP and FCM
  • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TCR V β 8.1-2 Antibody (KJ16). This work is in progress.
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    SEE ALSO...

    Additional TCR Antibodies including TCR α, TCR β, TCR C β 1, TCR δ, TCR γ, TCR V α 24 J α 18, TCR V α 8, TCR V β 11, TCR V β 13.1 and TCR V β 13.2

    TCR V β 8.1-2 Antibody (KJ16) is a rat monoclonal IgG antibody that detects the TCR V beta 8.1-2 protein of mouse origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). TCR V β 8.1-2 (KJ16) antibody is available in both non-conjugated and various conjugated forms, including phycoerythrin (PE) and fluorescein isothiocyanate (FITC), providing flexibility for different experimental needs. TCR V beta 8.1-2 protein plays a crucial role in the immune response by forming part of the T cell receptor, which is essential for recognizing foreign antigens. This recognition initiates a cascade of intracellular signals that activate T cells, enabling them to respond to infections and other immune challenges. The T cell receptor is a heterodimer composed of either alpha and beta or gamma and delta chains, with the majority of T cells (approximately 95%) expressing the alpha/beta heterodimer. The diversity of the TCR repertoire, which allows for the recognition of a wide array of antigens, is achieved through somatic recombination of variable (V), diversity (D), and joining (J) gene segments during T cell development. The genes encoding the TCR beta and gamma chains are located in distinct loci, while the alpha and delta chain genes are found within a single locus. Genetic mutations in the T cell receptor beta locus have been linked to the development of T cell lymphomas, highlighting the importance of this protein in both normal immune function and disease.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    TCR V β 8.1-2 Antibody (KJ16) References:

    1. T cell response in malaria pathogenesis: selective increase in T cells carrying the TCR V(beta)8 during experimental cerebral malaria.  |  Boubou, MI., et al. 1999. Int Immunol. 11: 1553-62. PMID: 10464176
    2. Therapy against murine collagen-induced arthritis with T cell receptor V beta-specific antibodies.  |  Chiocchia, G., et al. 1991. Eur J Immunol. 21: 2899-905. PMID: 1836185
    3. Expression of a murine polyclonal T cell receptor marker correlates with the use of specific members of the V beta 8 gene segment subfamily.  |  Behlke, MA., et al. 1987. J Exp Med. 165: 257-62. PMID: 2432150
    4. Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor.  |  Staerz, UD., et al. 1985. J Immunol. 134: 3994-4000. PMID: 2580901
    5. Methods for the selection and growth of antigen-specific cytolytic T lines and clones bearing a defined T cell receptor beta chain marker.  |  Ito, M., et al. 1987. J Immunol Methods. 103: 229-37. PMID: 2959725
    6. A mouse monoclonal antibody specific for the V beta 5.3 chain of the human TcR recognizes a subgroup of the mouse TcR V beta 8.2 chains.  |  Bleux, C., et al. 1995. J Leukoc Biol. 57: 491. PMID: 7884322
    7. Attenuation of lpr-graft-versus-host disease (GVHD) in MRL/lpr spleen cell-injected SCID mice by in vivo treatment with anti-V beta 8.1,2 monoclonal antibody.  |  Hosaka, N., et al. 1994. Clin Exp Immunol. 96: 500-7. PMID: 8004820
    8. Oligoclonality of T cells in salivary glands of autoimmune MRL/lpr mice.  |  Skarstein, K., et al. 1994. Immunology. 81: 497-501. PMID: 8039804
    9. Dominant V beta 8 gene usage in response to TNP: failure to use other V beta chains following removal of V beta 8+ T cells by monoclonal antibody in vivo.  |  Dieli, F., et al. 1994. Immunology. 82: 99-105. PMID: 8045598
    10. T cell vaccination against autoimmune diabetes in nonobese diabetic mice.  |  Formby, B. and Shao, T. 1993. Ann Clin Lab Sci. 23: 137-47. PMID: 8096128
    11. Involvement of TCR-V beta 8.3+ cells in the cure of mice bearing a large MOPC-315 tumor by low dose melphalan.  |  Mokyr, MB., et al. 1993. J Immunol. 151: 4838-46. PMID: 8409442
    12. Characterization of T cell receptor repertoire and anti-Ro/SSA autoantibodies in relation to sialadenitis of NOD mice.  |  Skarstein, K., et al. 1995. Autoimmunity. 22: 9-16. PMID: 8882417

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    TCR V β 8.1-2 Antibody (KJ16)

    sc-59387
    200 µg/ml
    		$322.00

    TCR V β 8.1-2 Antibody (KJ16)) FITC

    sc-59387 FITC
    200 µg/ml
    		$336.00

    TCR V β 8.1-2 Antibody (KJ16) PE

    sc-59387 PE
    200 µg/ml
    		$349.00

    How can TCR V β 8.1-2 (KJ16): sc-59387 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

    Asked by: Dr Ninau Qelp
    In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-59387 PE) or fluoroscein (sc-59387 FITC).
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 3 out of 5 by from Worked well for Western with D0Worked well for Western with D0-11.10 samples. -SCBT QC
    Date published: 2015-06-01
    Rated 3 out of 5 by from Nice WB results with T cell hybridomaNice WB results with T cell hybridoma, SKK-45.10. -SCBT QC
    Date published: 2013-06-25
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    TCR V β 8.1-2 Antibody (KJ16) is rated 3.0 out of 5 by 3.
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