TCR V β 4 Antibody (KT4): sc-53476

TCR V β 4 Antibody (KT4) is a rat monoclonal IgG2b antibody that detects the TCR V beta 4 protein of mouse origin by flow cytometry (FCM). TCR V β 4 (KT4) antibody is available in both non-conjugated and various conjugated forms, including phycoerythrin (PE) and fluorescein isothiocyanate (FITC), providing flexibility for different experimental needs. The T cell antigen receptor (TCR) plays a crucial role in the immune response by recognizing foreign antigens and initiating intracellular signaling that activates T cells, transitioning them from a resting state to an active one. The TCR is a heterodimer composed of either alpha and beta or gamma and delta chains, with approximately 95% of circulating T cells expressing the alpha/beta heterodimer. This diversity in T cell receptors is essential for the immune system′s ability to recognize a wide array of antigens, achieved through the somatic recombination of variable (V), diversity (D), and joining (J) gene segments during T cell development. The TCR beta and gamma chain genes are located in distinct loci, while the genes for the TCR alpha and delta chains are found within a single locus. Notably, the synthesis of the beta chain involves a complex process where a D segment is first joined with a J segment, followed by the addition of a V segment, and finally a constant (C) segment. Understanding the structure and function of the TCR is vital, as genetic mutations in the T cell receptor beta locus have been linked to T cell lymphomas, highlighting the importance of TCR V beta 4 in both normal immune function and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TCR V β 4 Antibody (KT4) References:

1. T cell receptor (TCR) signal strength controls arthritis severity in proteoglycan-specific TCR transgenic mice.  |  Olasz, K., et al. 2012. Clin Exp Immunol. 167: 346-55. PMID: 22236012
2. Expression of a murine polyclonal T cell receptor marker correlates with the use of specific members of the V beta 8 gene segment subfamily.  |  Behlke, MA., et al. 1987. J Exp Med. 165: 257-62. PMID: 2432150
3. Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor.  |  Staerz, UD., et al. 1985. J Immunol. 134: 3994-4000. PMID: 2580901
4. Methods for the selection and growth of antigen-specific cytolytic T lines and clones bearing a defined T cell receptor beta chain marker.  |  Ito, M., et al. 1987. J Immunol Methods. 103: 229-37. PMID: 2959725
5. Short-term administration of selected anti-T-cell receptor V beta chain specific MoAb reduces sialadenitis in MRL/lpr mice.  |  Skarstein, K., et al. 1995. Scand J Immunol. 42: 529-34. PMID: 7481557
6. Preferential but not exclusive T cell receptor V beta chain utilization of myelin basic protein and peptide-specific T cell clones in mice.  |  Kalman, B., et al. 1994. Cell Immunol. 153: 206-13. PMID: 7507001
7. Restricted expression of T cell receptor V beta and lymphokine genes in arthritic joints of a TCR V beta a (H-2q) mouse strain-BUB/BnJ-with collagen-induced arthritis.  |  Haqqi, TM., et al. 1995. Autoimmunity. 20: 163-70. PMID: 7578877
8. T cell receptor V beta repertoire in mice lacking endogenous mouse mammary tumor provirus.  |  Braun, MY., et al. 1995. Eur J Immunol. 25: 857-62. PMID: 7705418
9. A mouse monoclonal antibody specific for the V beta 5.3 chain of the human TcR recognizes a subgroup of the mouse TcR V beta 8.2 chains.  |  Bleux, C., et al. 1995. J Leukoc Biol. 57: 491. PMID: 7884322
10. Dominant V beta 8 gene usage in response to TNP: failure to use other V beta chains following removal of V beta 8+ T cells by monoclonal antibody in vivo.  |  Dieli, F., et al. 1994. Immunology. 82: 99-105. PMID: 8045598
11. T cell vaccination against autoimmune diabetes in nonobese diabetic mice.  |  Formby, B. and Shao, T. 1993. Ann Clin Lab Sci. 23: 137-47. PMID: 8096128
12. Involvement of TCR-V beta 8.3+ cells in the cure of mice bearing a large MOPC-315 tumor by low dose melphalan.  |  Mokyr, MB., et al. 1993. J Immunol. 151: 4838-46. PMID: 8409442

The preferred TCR β antibody is TCR β Antibody (G-11): sc-5277.