TCR α Antibody (H28-710): sc-101410

TCR α Antibody (H28-710) is a hamster monoclonal IgG2b antibody that detects TCR α protein of mouse origin by western blotting (WB) and immunoprecipitation (IP). TCR α Antibody (H28-710) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The T cell antigen receptor (TCR) plays a crucial role in the immune response by recognizing foreign antigens and initiating intracellular signaling that activates T cells, transitioning them from a resting state to an active one. The TCR is a heterodimer composed of either α and β or γ and δ chains, with approximately 95% of circulating T cells expressing the α/β heterodimer, while the remaining 2-5% express the γ/δ heterodimer. TCR functionality is enhanced by association with CD3 chains and the CD4 or CD8 co-receptors, which are essential for effective signal transduction. T helper cells, characterized by CD4 expression, and T cytotoxic cells, marked by CD8 expression, are vital components of the adaptive immune system. CD4 protein, a membrane glycoprotein with four extracellular immunoglobulin-like domains, increases the binding affinity between TCR and class II major histocompatibility complex (MHC) molecules presented by antigen-presenting cells, thereby facilitating a robust immune response.

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TCR α Antibody (H28-710) References:

1. Spontaneous chronic colitis in TCR alpha-mutant mice; an experimental model of human ulcerative colitis.  |  Bhan, AK., et al. 2000. Int Rev Immunol. 19: 123-38. PMID: 10723681
2. Regulation of TCR alpha and beta gene allelic exclusion during T-cell development.  |  Malissen, M., et al. 1992. Immunol Today. 13: 315-22. PMID: 1324691
3. Novel anti-CD4 monoclonal antibodies separate human immunodeficiency virus infection and fusion of CD4+ cells from virus binding.  |  Healey, D., et al. 1990. J Exp Med. 172: 1233-42. PMID: 1698911
4. The immunobiology of T cells with invariant gamma delta antigen receptors.  |  Allison, JP. and Havran, WL. 1991. Annu Rev Immunol. 9: 679-705. PMID: 1832874
5. Signal transduction by the T cell antigen receptor.  |  Weiss, A., et al. 1991. Semin Immunol. 3: 313-24. PMID: 1839225
6. Identification of the residues in human CD4 critical for the binding of HIV.  |  Arthos, J., et al. 1989. Cell. 57: 469-81. PMID: 2541915
7. Structure and expression of the human and mouse T4 genes.  |  Maddon, PJ., et al. 1987. Proc Natl Acad Sci U S A. 84: 9155-9. PMID: 3501122
8. Germline-like TCR-α chains shared between autoreactive T cells in blood and pancreas.  |  Linsley, PS., et al. 2024. Nat Commun. 15: 4971. PMID: 38871688
9. The interaction between CD4 and MHC class II molecules and its effect on T cell function.  |  Vignali, DA. 1994. Behring Inst Mitt. 133-47. PMID: 7998907
10. T cell receptor interaction with peptide/major histocompatibility complex (MHC) and superantigen/MHC ligands is dominated by antigen.  |  Ehrich, EW., et al. 1993. J Exp Med. 178: 713-22. PMID: 8393480
11. Distinct roles for CD4 and CD8 as co-receptors in antigen receptor signalling.  |  Julius, M., et al. 1993. Immunol Today. 14: 177-83. PMID: 8499078
12. Developmental regulation of V(D)J recombination at the TCR alpha/delta locus.  |  Krangel, MS., et al. 1998. Immunol Rev. 165: 131-47. PMID: 9850858

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TCR α Antibody (H28-710) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TCR α Antibody (H28-710) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TCR α Antibody (H28-710) Specifications

Alternate Product Name: T Cell Receptor

Product Clone Name: H28-710

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: TCR α

Epitope Species: mouse

Product Reactivity/Detection: TCR α

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TCR α Antibody (H28-710): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred TCR α antibody is TCR α Antibody (H-1): sc-515719.