TCP-1 α Antibody (91A): sc-53454

TCP-1 α Antibody (91A) is a rat monoclonal IgG2a antibody that detects TCP-1 alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). TCP-1 α (91A) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TCP-1, also known as t complex polypeptide 1, is a crucial subunit of the chaperonin containing TCP-1 (CCT) complex, which is essential for the proper folding of cytosolic proteins, particularly actins and tubulins. Located in the eukaryotic cytosol, the CCT complex comprises eight distinct subunits, each encoded by different genes, and is vital for maintaining cellular function and integrity. TCP-1′s role in assisting protein folding is particularly important, as misfolded proteins can lead to cellular dysfunction and contribute to various diseases. TCP-1 was initially identified in mice, where mutations were linked to tail-less and embryonic lethal phenotypes, and homologous sequences have been found in several other species, underscoring its evolutionary significance. The yeast version of TCP-1 acts as a molecular chaperone for actin and tubulin, highlighting the conserved nature of its function across different organisms.

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TCP-1 α Antibody (91A) References:

1. Upregulation of cytosolic chaperonin CCT subunits during recovery from chemical stress that causes accumulation of unfolded proteins.  |  Yokota, SI., et al. 2000. Eur J Biochem. 267: 1658-64. PMID: 10712596
2. Cloning of the chaperonin t-complex polypeptide 1 gene from Schistosoma mansoni and studies of its expression levels under heat shock and oxidative stress.  |  Campos, EG. and Hamdan, FF. 2000. Parasitol Res. 86: 253-8. PMID: 10726997
3. Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.  |  Hynes, GM. and Willison, KR. 2000. J Biol Chem. 275: 18985-94. PMID: 10748209
4. Defining the eukaryotic cytosolic chaperonin-binding sites in human tubulins.  |  Ritco-Vonsovici, M. and Willison, KR. 2000. J Mol Biol. 304: 81-98. PMID: 11071812
5. The signaling mechanism of Arabidopsis CRY1 involves direct interaction with COP1.  |  Yang, HQ., et al. 2001. Plant Cell. 13: 2573-87. PMID: 11752373
6. A mechanism for suppression of the CDP-choline pathway during apoptosis.  |  Morton, CC., et al. 2013. J Lipid Res. 54: 3373-84. PMID: 24136823
7. Nuclear-localized CTP:phosphocholine cytidylyltransferase α regulates phosphatidylcholine synthesis required for lipid droplet biogenesis.  |  Aitchison, AJ., et al. 2015. Mol Biol Cell. 26: 2927-38. PMID: 26108622
8. Accelerated phosphatidylcholine turnover in macrophages promotes adipose tissue inflammation in obesity.  |  Petkevicius, K., et al. 2019. Elife. 8: PMID: 31418690
9. Lipid-associated PML structures assemble nuclear lipid droplets containing CCTα and Lipin1.  |  Lee, J., et al. 2020. Life Sci Alliance. 3: PMID: 32461215
10. Combination of biomarkers for neoadjuvant systemic chemotherapy before cystectomy in patients with urinary bladder cancer.  |  Türker, P., et al. 2021. Transl Res. 235: 77-84. PMID: 33766713
11. Cucumber T-complex protein. Molecular cloning, bacterial expression and characterization within a 22-S cytosolic complex in cotyledons and hypocotyls.  |  Ahnert, V., et al. 1996. Eur J Biochem. 235: 114-9. PMID: 8631317
12. A Dictyostelium discoideum homologue to Tcp-1 is essential for growth and development.  |  Iijima, M., et al. 1998. Gene. 213: 101-6. PMID: 9630545

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TCP-1 α Antibody (91A) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-TCP-1 α Antibody (91A) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TCP-1 α Antibody (91A) Specifications

Alternate Product Name: TCP-1 α

Product Clone Name: 91A

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: TCP

Epitope Species: murine

Product Reactivity/Detection: TCP-1α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for TCP-1 α Antibody (91A): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin