Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TBL1XR1 CRISPR/Cas9 KO Plasmid (h): sc-401998
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TBL1XR1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TBL1XR1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TBL1XR1 Antibody (L-08): sc-100908
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TBL1XR1 CRISPR/Cas9 KO Plasmid (h) | sc-401998 | 20 µg | $397.00 |
Overview
TBL1XR1 (transducin β-like 1 X-linked receptor 1) encodes a WD40 repeat–containing coregulator that functions within NCoR/SMRT transcriptional repression complexes and participates in dynamic exchange between repression and activation at promoter and enhancer regions. It links chromatin-associated regulatory machinery to signal-dependent transcription programs, including pathways downstream of nuclear receptors and Wnt/β-catenin signaling, thereby influencing cell fate decisions, proliferation, and differentiation. Through its roles in transcriptional control and cofactor recruitment, TBL1XR1 has been implicated in oncogenic transcriptional states and in neurodevelopmental phenotypes reported in genetic studies. Perturbation of TBL1XR1 can therefore be used to interrogate chromatin-dependent gene regulation, transcriptional network stability, and pathway cross-talk in human cell models.
TBL1XR1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TBL1XR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TBL1XR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TBL1XR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TBL1XR1 protein expression.
This CRISPR knockout system enables efficient generation of TBL1XR1-deficient cell models for investigation of TBL1XR1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TBL1XR1 exon(s) critical for TBL1XR1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TBL1XR1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TBL1XR1 CRISPR/Cas9 KO Plasmid (h) and TBL1XR1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TBL1XR1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TBL1XR1 HDR Plasmid (h) and TBL1XR1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TBL1XR1 homology arms to support homology-directed repair at defined TBL1XR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.