Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TAUT CRISPR/Cas9 KO Plasmid (h): sc-402918
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TAUT CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TAUT genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TAUT Antibody (A-11): sc-393036
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAUT CRISPR/Cas9 KO Plasmid (h) | sc-402918 | 20 µg | $397.00 |
Overview
SLC6A6 encodes the taurine transporter TAUT, a sodium- and chloride-dependent membrane transporter that maintains intracellular taurine pools required for osmotic homeostasis, calcium handling, and cytoprotection against oxidative and ER stress. TAUT-mediated taurine uptake supports mitochondrial function, membrane stabilization, and signaling processes that influence cell survival, differentiation, and volume regulation across excitable and non-excitable tissues. Altered SLC6A6 activity and taurine availability have been linked to cellular stress susceptibility and dysfunction in systems such as retina, heart, kidney, and nervous tissue, making it relevant to studies of neurobiology, cardiometabolic regulation, and inflammatory stress responses. As part of solute carrier transport networks, TAUT intersects with pathways governing ion gradients, redox balance, and stress-adaptive gene programs.
TAUT CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC6A6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC6A6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC6A6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TAUT protein expression.
This CRISPR knockout system enables efficient generation of SLC6A6-deficient cell models for investigation of TAUT signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC6A6 exon(s) critical for TAUT function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC6A6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TAUT CRISPR/Cas9 KO Plasmid (h) and TAUT CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC6A6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TAUT HDR Plasmid (h) and TAUT HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC6A6 homology arms to support homology-directed repair at defined SLC6A6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.