Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TAP2 Double Nickase Plasmid (m): sc-423266-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TAP2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TAP2 Double Nickase Plasmid (m) and TAP2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Tap2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TAP2 Antibody (B-2): sc-515576
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAP2 Double Nickase Plasmid (m) | sc-423266-NIC | 20 µg | $410.00 | |||
TAP2 Double Nickase Plasmid (m2) | sc-423266-NIC-2 | 20 µg | $410.00 |
Mouse Tap2 encodes TAP2, an ATP-binding cassette transporter that heterodimerizes with TAP1 to translocate proteasome-derived peptides from the cytosol into the endoplasmic reticulum for loading onto MHC class I molecules. This transport step is central to antigen processing and presentation and integrates with proteasomal degradation, ER peptide loading, and immune surveillance pathways. Altered TAP2 activity can reshape the immunopeptidome and influence cell-surface MHC I expression, impacting studies of host–pathogen interactions, tumor immune evasion mechanisms, and immune-driven inflammation in experimental models. Tap2 is therefore widely used as a genetic node for dissecting interferon-responsive antigen presentation programs and T cell recognition determinants.
TAP2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Tap2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Tap2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Tap2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Tap2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.