TAP1 Antibody (B-8): sc-376796

TAP1 Antibody (B-8) is a mouse monoclonal IgG3 kappa light chain antibody that detects TAP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TAP1 (B-8) antibody is available as the non-conjugated form. The transporter associated with antigen processing (TAP) plays a crucial role in the immune response by facilitating peptide transport from the cytosol into the endoplasmic reticulum (ER) for loading onto major histocompatibility complex (MHC) class I molecules, which are essential for presenting antigens to CD8+ T cells. This process is vital for recognizing and eliminating infected or malignant cells, making TAP1 a key player in adaptive immunity. The TAP transporter consists of two subunits, TAP1 and TAP2, forming a dimeric structure that creates a translocation pore within the ER membrane. Each subunit contains a transmembrane region contributing to pore formation, as well as a cytoplasmic peptide-binding pocket ensuring selective peptide transport. Additionally, TAP1 and TAP2 possess nucleotide-binding domains that hydrolyze ATP, providing energy for conformational changes that facilitate peptide translocation. Proper functioning of TAP1 remains critical for effective immune surveillance and response, highlighting its significance in both basic immunology and potential therapeutic applications.

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TAP1 Antibody (B-8) References:

1. Nucleotide binding by TAP mediates association with peptide and release of assembled MHC class I molecules.  |  Knittler, MR., et al. 1999. Curr Biol. 9: 999-1008. PMID: 10508608
2. Membrane topology and dimerization of the two subunits of the transporter associated with antigen processing reveal a three-domain structure.  |  Vos, JC., et al. 1999. J Immunol. 163: 6679-85. PMID: 10586064
3. Pairing of the nucleotide binding domains of the transporter associated with antigen processing.  |  Lapinski, PE., et al. 2000. J Biol Chem. 275: 6831-40. PMID: 10702242
4. Characteristics of peptide and major histocompatibility complex class I/beta 2-microglobulin binding to the transporters associated with antigen processing (TAP1 and TAP2).  |  Androlewicz, MJ., et al. 1994. Proc Natl Acad Sci U S A. 91: 12716-20. PMID: 7809108
5. Evidence that transporters associated with antigen processing translocate a major histocompatibility complex class I-binding peptide into the endoplasmic reticulum in an ATP-dependent manner.  |  Androlewicz, MJ., et al. 1993. Proc Natl Acad Sci U S A. 90: 9130-4. PMID: 8415666
6. Multiple regions of the transporter associated with antigen processing (TAP) contribute to its peptide binding site.  |  Nijenhuis, M. and Hämmerling, GJ. 1996. J Immunol. 157: 5467-77. PMID: 8955196
7. Major histocompatibility complex class I molecules interact with both subunits of the transporter associated with antigen processing, TAP1 and TAP2.  |  Powis, SJ. 1997. Eur J Immunol. 27: 2744-7. PMID: 9368636

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TAP1 Antibody (B-8) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-TAP1 Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TAP1 Antibody (B-8) Specifications

Alternate Product Name: TAP1

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 697-724

Epitope Target: TAP1

Epitope Species: mouse

Product Reactivity/Detection: all TAP1 isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for TAP1 Antibody (B-8): P: Blocking Peptide

The preferred TAP1 antibody is TAP1 Antibody (D-11): sc-518133.