Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TALL-1 CRISPR/Cas9 KO Plasmid (h): sc-406502
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TALL-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TALL-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TALL-1 Antibody (6F2): sc-32773
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TALL-1 CRISPR/Cas9 KO Plasmid (h) | sc-406502 | 20 µg | $397.00 |
Overview
TNFSF13B encodes TALL-1 (also known as BAFF), a TNF superfamily cytokine essential for B cell survival, maturation, and immunoglobulin production. TALL-1 signals through receptors including BAFF-R, TACI, and BCMA to activate NF-κB and related survival pathways, shaping peripheral B cell homeostasis and humoral immune responses. Dysregulated TNFSF13B expression or signaling is associated with altered B cell tolerance and aberrant plasma cell persistence, linking this axis to autoimmune-associated immune activation and B cell–driven inflammatory states. As a soluble and membrane-associated ligand, TALL-1 also influences immune cell crosstalk within lymphoid tissues and inflamed microenvironments.
TALL-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TNFSF13B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TNFSF13B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TNFSF13B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TALL-1 protein expression.
This CRISPR knockout system enables efficient generation of TNFSF13B-deficient cell models for investigation of TALL-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TNFSF13B exon(s) critical for TALL-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TNFSF13B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TALL-1 CRISPR/Cas9 KO Plasmid (h) and TALL-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TNFSF13B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TALL-1 HDR Plasmid (h) and TALL-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TNFSF13B homology arms to support homology-directed repair at defined TNFSF13B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.