TAF II p250 CRISPR/Cas9 KO Plasmid (m): sc-435426

Overview

Taf1 encodes TAF II p250 (TAF1), a core subunit of the TFIID complex that nucleates RNA polymerase II preinitiation complex assembly and integrates promoter recognition with transcriptional output. As a multifunctional regulator with kinase and acetyltransferase activities, TAF1 influences chromatin organization, transcription initiation, and cell cycle–linked gene expression programs. In mouse systems, Taf1-dependent transcriptional control is central to embryonic development, neuronal gene regulation, and proteostasis pathways that shape cellular stress responses. Dysregulation of TAF1 and TFIID-mediated transcription has been linked to neurodevelopmental phenotypes and broader perturbations of gene expression homeostasis relevant to disease modeling.

TAF II p250 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Taf1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Taf1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Taf1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TAF II p250 protein expression.

This CRISPR knockout system enables efficient generation of Taf1-deficient cell models for investigation of TAF II p250 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Taf1 exon(s) critical for TAF II p250 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Taf1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by TAF II p250 CRISPR/Cas9 KO Plasmid (m) and TAF II p250 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Taf1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by TAF II p250 HDR Plasmid (m) and TAF II p250 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Taf1 homology arms to support homology-directed repair at defined Taf1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.