Anti-TAF I p110 Antibody (C-10) recommended for detection of TAF I p110 of human origin by WB, IP, IF and ELISA

Anti-TAF I p110 Antibody (C-10): sc-374551

Anti-TAF I p110 Antibody (C-10) is rated 4.5 out of 5 by 2.
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Datasheets

    • Anti-TAF I p110 Antibody (C-10) is a mouse monoclonal IgG1 (kappa light chain) TAF I p110 antibody provided at 200 µg/ml
    • raised against amino acids 570-869 mapping at the C-terminus of TAF I p110 of human origin
    • TAF I p110 Antibody (C-10) is recommended for detection of TAF I p110 of human origin by WB, IP, IF and ELISA
    • Anti-TAF I p110 Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-374551 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TAF I p110 (C-10): sc-374551.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TAF I p110 Antibody (C-10) for WB applications. These reagents are now offered in bundles with TAF I p110 Antibody (C-10) (see ordering information below).
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What is the recommended starting dilution for Western blot for TAF I p110 (C-10): sc-374551 monoclonal antibody?

Asked by: AbPolly
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-374551.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from an excellent antibody for WB This antibody recognizes a band of good signal at the predicted M.W on the WB (1:500 dilution, 40 ug HeLa lysate). However, it does not appear to stain the nuclei in formaldehyde-fixed HeLa cells
Date published: 2017-07-30
Rated 5 out of 5 by from Produced nice Western blot data of TAF I Produced nice Western blot data of TAF I p110 expression in PC-3 nuclear extract and HT-1080 whole cell lysate . -SCBT QC
Date published: 2014-08-28
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