Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TADA2B Double Nickase Plasmid (h): sc-406224-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TADA2B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TADA2B Double Nickase Plasmid (h) and TADA2B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TADA2B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TADA2B Antibody (MB-56): sc-130479
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TADA2B Double Nickase Plasmid (h) | sc-406224-NIC | 20 µg | $410.00 | |||
TADA2B Double Nickase Plasmid (h2) | sc-406224-NIC-2 | 20 µg | $410.00 |
TADA2B encodes a transcriptional adaptor component of the SAGA/ATAC histone acetyltransferase coactivator complexes, linking sequence-specific transcription factors to chromatin-modifying machinery. Through interactions with GCN5/PCAF and related subunits, TADA2B contributes to histone acetylation, promoter accessibility, and coordinated regulation of gene expression programs involved in cell cycle control, differentiation, and responses to cellular stress. Perturbation of SAGA/ATAC complex integrity can disrupt epigenetic regulation and transcriptional homeostasis, processes frequently implicated in oncogenic transformation and other disorders of gene regulation. As a chromatin-associated regulator, TADA2B is relevant for studying mechanisms that couple histone acetylation to transcription initiation and enhancer–promoter communication in human cells.
TADA2B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TADA2B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TADA2B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TADA2B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TADA2B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.