Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
T6BP CRISPR/Cas9 KO Plasmid (h): sc-404858
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- T6BP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the T6BP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: T6BP Antibody (H-6): sc-393143
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
T6BP CRISPR/Cas9 KO Plasmid (h) | sc-404858 | 20 µg | $397.00 |
Overview
TAX1BP1 encodes T6BP, an adaptor protein that coordinates ubiquitin-dependent signaling at the intersection of innate immunity, inflammatory control, and selective autophagy. T6BP binds ubiquitin chains and partners such as A20/TNFAIP3 and TRAF proteins to modulate NF-κB signaling downstream of receptors including TNFR and Toll-like receptors, helping tune cytokine and antiviral responses. It also participates in autophagic handling of ubiquitinated cargo and quality control pathways that influence cellular stress tolerance and survival. Dysregulation of TAX1BP1-linked signaling and proteostasis has been associated with inflammatory phenotypes and cancer-relevant pathways, making it a useful node for mechanistic studies of immune regulation and ubiquitin signaling.
T6BP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TAX1BP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TAX1BP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TAX1BP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish T6BP protein expression.
This CRISPR knockout system enables efficient generation of TAX1BP1-deficient cell models for investigation of T6BP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TAX1BP1 exon(s) critical for T6BP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TAX1BP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by T6BP CRISPR/Cas9 KO Plasmid (h) and T6BP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TAX1BP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by T6BP HDR Plasmid (h) and T6BP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TAX1BP1 homology arms to support homology-directed repair at defined TAX1BP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.