T2R38 CRISPR/Cas9 KO Plasmid (h): sc-402279
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- T2R38 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the T2R38 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
T2R38 CRISPR/Cas9 KO Plasmid (h) | sc-402279 | 20 µg | $397.00 |
Overview
TAS2R38 encodes the bitter taste receptor T2R38, a class A GPCR that detects thiourea-containing compounds such as PTC/PROP and couples to gustducin-associated signaling to modulate intracellular second messengers, including Ca²⁺ flux, in chemosensory cells. Beyond oral taste perception, T2R38 is expressed in extraoral epithelia where bitter receptor signaling contributes to chemosensory surveillance and regulation of epithelial responses. Common TAS2R38 haplotypes alter receptor sensitivity and have been studied as genetic determinants of bitter taste perception and variability in chemosensory-driven behaviors. As a GPCR-linked sensory receptor, T2R38 provides a tractable model for dissecting receptor signaling, expression control, and genotype–phenotype relationships in human cell systems.
T2R38 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TAS2R38 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TAS2R38 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TAS2R38 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish T2R38 protein expression.
This CRISPR knockout system enables efficient generation of TAS2R38-deficient cell models for investigation of T2R38 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TAS2R38 exon(s) critical for T2R38 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TAS2R38 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by T2R38 CRISPR/Cas9 KO Plasmid (h) and T2R38 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TAS2R38 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by T2R38 HDR Plasmid (h) and T2R38 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TAS2R38 homology arms to support homology-directed repair at defined TAS2R38 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.