T-FABP CRISPR Plasmids (m)

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Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number
Mouse	Fabp9	21884	3 A1	NM_011598	O08716	N/A

Description

20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
T-FABP CRISPR/Cas9 Knockout (KO) Plasmid (m) consists of a pool of three plasmids each encoding the Cas9 nuclease and a T-FABP-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency
gRNA sequences are derived from the GeCKO (v2) library and direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
**** NOTE: No corresponding HDR is available for this Knockout Plasmid. **** Please use sc-423411-KO-2 and sc-423411-HDR-2 if selection of cells using puromycin resistance is desired.
Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: T-FABP Antibody (B-4): sc-390196

See Protocol Online.

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Description

20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
T-FABP Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
The paired gRNAs encoded by T-FABP Double Nickase Plasmid (m2) differ from the paired gRNAs in T-FABP Double Nickase Plasmid (m)
Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: T-FABP Antibody (B-4): sc-390196

See Protocol Online.

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Description

20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
T-FABP CRISPR Activation Plasmid (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
T-FABP CRISPR Activation Plasmid (m) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: T-FABP Antibody (B-4): sc-390196

See Protocol Online.

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Description

200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
T-FABP Lentiviral Activation Particles (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
T-FABP Lentiviral Activation Particles (m) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt. guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt. upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
The guide RNA in the T-FABP Lentiviral Activation Particles (m2) encodes a target-specific 20 nt guide RNA that differs from the guide RNA in T-FABP Lentiviral Activation Particles (m)
Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: T-FABP Antibody (B-4): sc-390196

See Protocol Online.

DOWNLOADS

QUICK LINKS