SZT2 CRISPR/Cas9 KO Plasmid (h): sc-411718
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SZT2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SZT2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SZT2 CRISPR/Cas9 KO Plasmid (h) | sc-411718 | 20 µg | $397.00 |
Overview
SZT2 (seizure threshold 2) encodes a large cytosolic protein that functions as a negative regulator of mTORC1 signaling through the KICSTOR complex, linking nutrient and amino acid availability to lysosomal control of mTORC1 activity. By constraining mTORC1 activation, SZT2 helps maintain cellular homeostasis across processes such as autophagy, metabolism, and growth signaling. Disruption of SZT2 has been associated with neurodevelopmental phenotypes and epileptic encephalopathies, consistent with altered nutrient sensing and mTOR pathway dysregulation in neurons. As a result, SZT2 is frequently investigated in models of neuronal excitability, lysosome-associated signaling, and mTORC1-dependent stress responses.
SZT2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SZT2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SZT2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SZT2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SZT2 protein expression.
This CRISPR knockout system enables efficient generation of SZT2-deficient cell models for investigation of SZT2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SZT2 exon(s) critical for SZT2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SZT2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SZT2 CRISPR/Cas9 KO Plasmid (h) and SZT2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SZT2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SZT2 HDR Plasmid (h) and SZT2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SZT2 homology arms to support homology-directed repair at defined SZT2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.