SYP/Synaptophysin Antibody (2): sc-136271

SYP Antibody (2) is a mouse monoclonal IgG1 antibody that detects synaptophysin (SYP) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). SYP is an integral membrane protein predominantly found in neurons and neuroendocrine cells, where SYP plays a crucial role in the formation and recycling of synaptic vesicles. SYP is characterized by N-glycosylation and the ability to form hexameric complexes, which are essential for function as a large conductance channel. SYP contains four transmembrane domains, allowing integration into the membrane and potential function similar to gap junctions, facilitating intercellular communication. SYP interacts with membrane cholesterol, which is vital for vesicle formation, and binds to synaptobrevin (VAMP), a protein upregulated during neuronal development. While the synaptophysin-synaptobrevin complex is not essential for the exocytosis process, this complex serves as a reservoir of synaptobrevin, ensuring sufficient levels are available for neurotransmitter release. Additionally, the tail domain of brain Myosin V forms a stable complex with synaptobrevin II and SYP, which disassembles upon calcium ion influx during neuronal depolarization, highlighting the dynamic nature of these interactions in synaptic function. Anti-SYP antibody (2) is an invaluable tool for researchers studying synaptic mechanisms and synaptophysin′s role in neuronal communication.

The preferred SYP/Synaptophysin antibody is SYP/Synaptophysin Antibody (D-4): sc-17750.