Syntaxin 1 Antibody (SP8): sc-58299
- Syntaxin 1 Antibody (SP8) is a mouse monoclonal IgG1 κ, cited in 5 publications, provided at 200 µg/ml
- raised against a crude synaptic preparation from human postmortem brain
- recommended for detection of native, denatured and recombinant presynaptic membrane protein Syntaxin 1 (isoform 1A, amino acids 4-190) expressed in bacterial systems of mouse, rat, human and porcine origin by WB, IP and IF
- See Syntaxin 1 (HPC-1): sc-12736 for Syntaxin 1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Syntaxin 1 Antibody (SP8) for WB applications. This reagent is now offered in a bundle with Syntaxin 1 Antibody (SP8) (see ordering information below).
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Syntaxin 1 Antibody (SP8) is a mouse monoclonal IgG1 antibody that detects Syntaxin 1 in mouse, rat, human, and porcine samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Syntaxin 1 plays a crucial role in vesicular transport, vital for proper functioning of eukaryotic cells. Syntaxin 1 belongs to the SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein REceptor) family, essential for synaptic vesicle fusion with plasma membrane, facilitating neurotransmitter release. Syntaxin 1 is primarily located in presynaptic terminals of neurons, where anti-Syntaxin 1 antibody (SP8) can detect interactions with other SNARE proteins, including VAMP and SNAP-25, forming a stable SNARE complex. This complex brings vesicle and target membrane into close proximity, driving lipid bilayer fusion. Proper functioning of Syntaxin 1 is critical for synaptic transmission and neuronal communication, and dysregulation has been implicated in various neurological disorders. Syntaxin 1 (SP8) monoclonal antibody provides a reliable tool for researchers studying dynamics of synaptic vesicle fusion and underlying mechanisms of synaptic transmission.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Syntaxin 1 Antibody (SP8) References:
- Co-expression of several human syntaxin genes in neutrophils and differentiating HL-60 cells: variant isoforms and detection of syntaxin 1. | Martín-Martín, B., et al. 1999. J Leukoc Biol. 65: 397-406. PMID: 10080545
- Calcium- and syntaxin 1-mediated trafficking of the neuronal glycine transporter GLYT2. | Geerlings, A., et al. 2001. J Biol Chem. 276: 17584-90. PMID: 11278707
- Staring, a novel E3 ubiquitin-protein ligase that targets syntaxin 1 for degradation. | Chin, LS., et al. 2002. J Biol Chem. 277: 35071-9. PMID: 12121982
- Ectopic expression of syntaxin 1 in the ER redirects TI-VAMP- and cellubrevin-containing vesicles. | Martinez-Arca, S., et al. 2003. J Cell Sci. 116: 2805-16. PMID: 12759369
- Cellular localization and stimulation-associated distribution dynamics of syntaxin-1 and syntaxin-3 in gastric parietal cells. | Karvar, S., et al. 2005. Traffic. 6: 654-66. PMID: 15998321
- Phosphorylation of adult type Sept5 (CDCrel-1) by cyclin-dependent kinase 5 inhibits interaction with syntaxin-1. | Taniguchi, M., et al. 2007. J Biol Chem. 282: 7869-76. PMID: 17224448
- Deficits in syntaxin 1 phosphorylation in schizophrenia prefrontal cortex. | Castillo, MA., et al. 2010. Biol Psychiatry. 67: 208-16. PMID: 19748077
- VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex. | Benagiano, V., et al. 2011. BMC Neurosci. 12: 118. PMID: 22094010
- Phosphorylation-regulated axonal dependent transport of syntaxin 1 is mediated by a Kinesin-1 adapter. | Chua, JJ., et al. 2012. Proc Natl Acad Sci U S A. 109: 5862-7. PMID: 22451907
- Mislocalization of syntaxin-1 and impaired neurite growth observed in a human iPSC model for STXBP1-related epileptic encephalopathy. | Yamashita, S., et al. 2016. Epilepsia. 57: e81-6. PMID: 26918652
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Syntaxin 1 Antibody (SP8) | sc-58299 | 200 µg/ml | $322.00 | |||
Syntaxin 1 Antibody (SP8): m-IgG Fc BP-HRP Bundle | sc-551943 | 200 µg Ab; 10 µg BP | $361.00 |