Syntaxin 1 Antibody (SP5): sc-65509

Syntaxin 1 Antibody (SP5) is a mouse monoclonal IgG1 antibody that detects Syntaxin 1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Syntaxin 1 plays a crucial role in vesicular transport, which is vital for proper functioning of eukaryotic cells. Syntaxin 1 is primarily located on the plasma membrane and is essential for fusion of synaptic vesicles with the membrane, facilitating neurotransmitter release in neurons. Syntaxin 1′s presence at the synapse is critical for effective communication between nerve cells. Additionally, Syntaxin 1 interacts with other proteins, including VAMP and SNAP-25, to form the SNARE complex, which is necessary for fusion of vesicles with target membranes. This interaction is fundamental for neurotransmission and other cellular processes, highlighting Syntaxin 1′s significance in maintaining cellular homeostasis and signaling. Anti-Syntaxin 1 antibody (SP5) is an invaluable tool for researchers studying synaptic function and vesicular transport mechanisms.

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Syntaxin 1 Antibody (SP5) References:

1. Phosphorylated syntaxin 1 is localized to discrete domains along a subset of axons.  |  Foletti, DL., et al. 2000. J Neurosci. 20: 4535-44. PMID: 10844023
2. Differential contribution of syntaxin 1 and SNAP-25 to secretion in noradrenergic and adrenergic chromaffin cells.  |  Baltazar, G., et al. 2000. Eur J Cell Biol. 79: 883-91. PMID: 11152280
3. Correlation of syntaxin-1 and SNAP-25 clusters with docking and fusion of insulin granules analysed by total internal reflection fluorescence microscopy.  |  Ohara-Imaizumi, M., et al. 2004. Diabetologia. 47: 2200-7. PMID: 15647897
4. Identification and characterization of syntaxin 1 antisense variants in Limulus polyphemus.  |  Cao, Z., et al. 2006. Cell Mol Neurobiol. 26: 53-66. PMID: 16633901
5. The testis-specific VAD1.3/AEP1 interacts with β-actin and syntaxin 1 and directs peri-nuclear/Golgi expression with bipartite nucleus localization (BNL) sequence.  |  Zuo, Y., et al. 2010. Biochem Biophys Res Commun. 401: 275-80. PMID: 20850414
6. PRIP (phospholipase C-related but catalytically inactive protein) inhibits exocytosis by direct interactions with syntaxin 1 and SNAP-25 through its C2 domain.  |  Zhang, Z., et al. 2013. J Biol Chem. 288: 7769-7780. PMID: 23341457
7. Cytotoxicity of botulinum neurotoxins reveals a direct role of syntaxin 1 and SNAP-25 in neuron survival.  |  Peng, L., et al. 2013. Nat Commun. 4: 1472. PMID: 23403573
8. Rare autism-associated variants implicate syntaxin 1 (STX1 R26Q) phosphorylation and the dopamine transporter (hDAT R51W) in dopamine neurotransmission and behaviors.  |  Cartier, E., et al. 2015. EBioMedicine. 2: 135-146. PMID: 25774383
9. Molecular characterization and gene expression of syntaxin-1 and VAMP2 in the olfactory organ and brain during both seaward and homeward migrations of chum salmon, Oncorhynchus keta.  |  Abe, T. and Kudo, H. 2019. Comp Biochem Physiol A Mol Integr Physiol. 227: 39-50. PMID: 30236910
10. Membrane-mediated disorder-to-order transition of SNAP25 flexible linker facilitates its interaction with syntaxin-1 and SNARE-complex assembly.  |  Jiang, X., et al. 2019. FASEB J. 33: 7985-7994. PMID: 30916996

The preferred Syntaxin 1 antibody is Syntaxin 1 Antibody (HPC-1): sc-12736.