Synoviolin Double Nickase Plasmid (h): sc-403476-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Synoviolin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Synoviolin Double Nickase Plasmid (h) and Synoviolin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SYVN1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Synoviolin Antibody (4H4): sc-293484
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Synoviolin Double Nickase Plasmid (h) | sc-403476-NIC | 20 µg | $410.00 | |||
Synoviolin Double Nickase Plasmid (h2) | sc-403476-NIC-2 | 20 µg | $410.00 |
SYVN1 encodes synoviolin, an endoplasmic reticulum (ER)–resident RING-type E3 ubiquitin ligase that functions in ER-associated degradation (ERAD) by ubiquitinating misfolded or surplus proteins for proteasomal turnover. Through modulation of ubiquitin-dependent protein quality control, synoviolin influences ER proteostasis, cellular stress responses, and inflammatory signaling networks. Altered SYVN1 activity has been linked to dysregulated ER stress handling and aberrant protein homeostasis in contexts including synovial hyperplasia and other inflammation-associated pathologies. As a regulator of ubiquitination and ERAD, SYVN1 is widely studied in mechanisms connecting proteostasis to cell survival, immune signaling, and tissue remodeling.
Synoviolin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SYVN1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SYVN1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SYVN1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SYVN1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.