Synapsin Ia/b Antibody (B-11) recommended for detection of a broad range of synapsin family memebers of mouse, rat and human origin by WB, IP, IF and ELISA

Synapsin Ia/b Antibody (B-11): sc-376622

Synapsin Ia/b Antibody (B-11) is rated 5.0 out of 5 by 3.
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Datasheets

    • Synapsin Ia/b Antibody (B-11) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-29 at the N-terminus of Synapsin Ia/b of human origin
    • recommended for detection of a broad range of synapsin family memebers of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
    • blocking peptide, sc-376622 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

What application is the blocking peptide sc-376622 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Clean detection of Synapsin I a/b This antibody detects both Synapsin I a/b and Synapsin II in rat neuronal culture lysate at a 1:1000 dilution. Clean background and decent signal for both proteins. Published western blotting data using these antibodies.
Date published: 2018-04-06
Rated 5 out of 5 by from It works well in western blot I bought this antibody four months ago, and used it to detect the synapsin-1 expression in mouse cortex and hippocampus tissue. The conditions were as followed: 1:1000 diluted in 1xPBST buffer, 4℃ for 10 hours. The result was very good, bands were clear, both a and b isoform canbe detected.
Date published: 2017-04-26
Rated 5 out of 5 by from Great Western blot data of Synapsin Ia/b Great Western blot data of Synapsin Ia/b expression in non-transfected and mouse Synapsin Ia/b transfected 293T whole cell lysates and rat brain tissue extract . -SCBT QC
Date published: 2013-02-24
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