Synapsin Ia/b Antibody (A-8) recommended for detection of a broad range of synapsin family members of mouse, rat and human origin by WB, IP, IF and ELISA

Synapsin Ia/b Antibody (A-8): sc-376623

Synapsin Ia/b Antibody (A-8) is rated 4.8 out of 5 by 11.
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Datasheets

    • Anti-Synapsin Ia/b Antibody (A-8) is a mouse monoclonal IgM (kappa light chain) Synapsin Ia/b antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-29 at the N-terminus of Synapsin Ia/b of human origin
    • recommended for detection of a broad range of synapsin family members of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
    • blocking peptide, sc-376623 P
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Synapsin Ia/b (A-8): sc-376623.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

What application is the blocking peptide sc-376623 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Perfect staining at low concentration FEEDBACK ABOUT SANTA CRUZ sc-376623 SAMPLE The sample sc-376623 has been used with the following protocol: sc-376623 1:600 in phosphate buffer 0.1% (PB)+ bovin serum albumin (BSA) 10% + normal donkey serum (NDS) 0.1%. Overnight incubation on free floating slices at room temperature (RT), followed by washings and incubation with secondary Ab, 2 h at RT. Tissue was coronal mouse 40 µm brain slices comprehending hippocampus, transcardially purfused with 0.9% saline in PB and fixed with 4% paraformaldehyde in PB (PAF), post fixed with the same overnight and cryoprotected with 30% sucrose in PB after washing from PAF. Imagines have been taken with a confocal microscope with the following assembly: Crisel Instrument with spinning disk (X-light V2, Crest Optics, Italy) equipped with 40X oil immersion obj (Nikon, Japan), LED light source(Lumencor Spectra X,), with bandpass excitation filters of 460-490 and 535-600 nm (Chroma Technology, US). My feedback is perfect staining. Concentration may be reduced for the same protocol.
Date published: 2019-04-05
Rated 4 out of 5 by from Good Antibody I tested this antibody on mouse brain lysates, detected two bands (approx. 60 kDa and 90kda) in WB. I used
Date published: 2018-07-06
Rated 5 out of 5 by from Good antibody for WB I used this antibody in WB. It is strong and clear with clean background.
Date published: 2017-09-09
Rated 5 out of 5 by from 抗体特异性高 上星期我申请了该抗体的小样试用装,用其检测SD大鼠海马体细胞synapsin蛋白的表达量,western blot结果显示该抗体能够满足实验需要,并且特异性好。
Date published: 2017-09-05
Rated 5 out of 5 by from Great antibody for WB Great antibody for WB, I got strong band and clear background.
Date published: 2017-03-23
Rated 5 out of 5 by from Great monoclonal antibody Synapsin Ia/b Antibody (A-8) great for Western blot and IF. Nice strong band and specific robust stain
Date published: 2017-02-09
Rated 5 out of 5 by from Highly recommended I introduced this antibody in my field literature since the 2010. Our whole lab uses it as well as our collaborators in Europe.
Date published: 2016-12-17
Rated 5 out of 5 by from Good results This antibody works well in rat cortex protein extraction using 1:1000 dilution
Date published: 2016-12-12
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