symplekin Antibody (YI-9): sc-101128
- symplekin Antibody (YI-9) is a mouse monoclonal mouse IgG1 κ κ provided at 100 µg/ml
- raised against recombinant symplekin of human origin
- recommended for detection of symplekin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for symplekin Antibody (YI-9). This work is in progress.
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Symplekin Antibody (YI-9) is a mouse monoclonal IgG1 antibody that recognizes symplekin in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Symplekin is a crucial regulatory protein that plays a significant role in mRNA polyadenylation, essential for gene expression and mRNA stability. Symplekin is predominantly located in the nucleus, cytoplasm, cytoskeleton, and cell junctions, highlighting versatile functions within the cell. Symplekin presence in diverse tissues such as testis, pancreas, stomach, liver, and fetal brain underscores importance in various biological processes. Anti-symplekin antibody (YI-9) recognizes a protein that forms a high molecular weight complex, referred to as the tight junction complex, with other proteins involved in polyadenylation, acting as a scaffold that recruits regulatory proteins to specific sites. Symplekin is involved in the 3′-end maturation of histone mRNAs, vital for proper gene regulation during cell division. Symplekin gene defects have been linked to hepatocellular carcinoma, suggesting a role in tumorigenesis. Symplekin is expressed in two isoforms due to alternative splicing, contributing to functional diversity in cellular processes.
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symplekin Antibody (YI-9) References:
- Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation. | Barnard, DC., et al. 2004. Cell. 119: 641-51. PMID: 15550246
- Symplekin and multiple other polyadenylation factors participate in 3'-end maturation of histone mRNAs. | Kolev, NG. and Steitz, JA. 2005. Genes Dev. 19: 2583-92. PMID: 16230528
- The symplekin/ZONAB complex inhibits intestinal cell differentiation by the repression of AML1/Runx1. | Buchert, M., et al. 2009. Gastroenterology. 137: 156-64, 164.e1-3. PMID: 19328795
- Symplekin promotes tumorigenicity by up-regulating claudin-2 expression. | Buchert, M., et al. 2010. Proc Natl Acad Sci U S A. 107: 2628-33. PMID: 20133805
- Symplekin specifies mitotic fidelity by supporting microtubule dynamics. | Cappell, KM., et al. 2010. Mol Cell Biol. 30: 5135-44. PMID: 20823274
- Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction. | Chang, H., et al. 2012. Histochem Cell Biol. 137: 319-27. PMID: 22218735
- Symplekin, a polyadenylation factor, prevents MOZ and MLL activity on HOXA9 in hematopoietic cells. | Largeot, A., et al. 2013. Biochim Biophys Acta. 1833: 3054-3063. PMID: 23994619
- Nuclear accumulation of symplekin promotes cellular proliferation and dedifferentiation in an ERK1/2-dependent manner. | Zhang, C., et al. 2017. Sci Rep. 7: 3769. PMID: 28630428
- Molecular details of the CPSF73-CPSF100 C-terminal heterodimer and interaction with Symplekin. | Thore, S., et al. 2023. Open Biol. 13: 230221. PMID: 37989222
- Restrictor synergizes with Symplekin and PNUTS to terminate extragenic transcription. | Russo, M., et al. 2023. Genes Dev. 37: 1017-1040. PMID: 38092518
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
symplekin Antibody (YI-9) | sc-101128 | 100 µg/ml | $339.00 |