Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Syk Double Nickase Plasmid (h): sc-400189-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Syk Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Syk Double Nickase Plasmid (h) and Syk Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SYK. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Syk Antibody (4D10): sc-1240
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Syk Double Nickase Plasmid (h) | sc-400189-NIC | 20 µg | $410.00 | |||
Syk Double Nickase Plasmid (h2) | sc-400189-NIC-2 | 20 µg | $410.00 |
SYK encodes Syk, a cytosolic non-receptor tyrosine kinase that couples immunoreceptor signaling to downstream effector pathways in hematopoietic cells. Upon engagement of ITAM-containing receptors such as the B cell receptor and Fc receptors, Syk phosphorylates adaptor proteins and enzymes to propagate signaling through PLCγ, PI3K–AKT, MAPK/ERK, and NF-κB, shaping calcium flux, transcriptional programs, proliferation, and cytokine responses. Beyond immune activation, Syk contributes to FcR-mediated phagocytosis, inflammasome-related signaling contexts, and cytoskeletal remodeling via integrin-linked pathways. Dysregulated SYK signaling has been implicated in aberrant B cell activation and inflammatory circuitries, making it a frequent focus for studies of immune signaling networks and hematologic disease mechanisms.
Syk Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SYK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SYK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SYK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SYK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.