Syk Antibody (6A464): sc-73089

Syk Antibody (6A464) is a mouse monoclonal IgG1 antibody that detects Syk in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Syk, or spleen tyrosine kinase, is a 635 amino acid protein characterized by one protein kinase domain and two SH2 domains, playing a crucial role as a positive effector in B cell antigen receptor (CD79)-stimulated responses. Syk is essential for coupling CD79 with calcium ion movement through two phospho-regulated pathways, which are vital for various cellular processes. Notably, when Syk is phosphorylated on tyrosine residues 348 and 352, Syk activates the phospholipase C gamma-dependent pathway, while in an unphosphorylated state, Syk engages the phosphoinositide 3-kinase-dependent pathway. The regulation of calcium ion flow is critical for processes such as differentiation, phagocytosis, proliferation, and B cell development. Furthermore, Syk expression is notably upregulated in T cell lymphoma, indicating potential involvement in tumorigenesis. The existence of two isoforms of Syk, short and long, resulting from alternative splicing events, adds another layer of complexity to functional diversity. Anti-Syk antibody (6A464) is an invaluable tool for researchers investigating the intricate roles of Syk in cellular signaling and disease mechanisms.

The preferred Syk antibody is Syk Antibody (4D10): sc-1240.