SWAP-70 Double Nickase Plasmid (h2): sc-403465-NIC-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SWAP-70 Double Nickase Plasmid (h2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SWAP-70 Double Nickase Plasmid (h2) and SWAP-70 Double Nickase Plasmid (h22) encode distinct paired gRNA designs targeting SWAP70. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SWAP-70 Antibody (F-3): sc-390431
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SWAP-70 Double Nickase Plasmid (h2) | sc-403465-NIC-2 | 20 µg | $410.00 |
Human SWAP70 encodes SWAP-70, a phosphatidylinositol-binding signaling adaptor that is recruited to activated membranes downstream of PI3K and supports Rho-family GTPase–dependent actin cytoskeleton remodeling. SWAP-70 contributes to immune cell activation, adhesion, and migration by coordinating cytoskeletal dynamics and receptor-driven signaling in processes such as B cell responses, antigen-dependent trafficking, and phagocytic functions. Dysregulation of SWAP70-linked pathways has been investigated in the context of immune dysfunction and tumor cell motility/invasion, where altered actin remodeling can influence cellular behavior. SWAP70 perturbation models enable mechanistic studies of PI3K–Rac/Rho signaling, membrane trafficking, and cytoskeleton-controlled phenotypes using gene editing to interrogate effects on migration, morphology, and stimulus-dependent signaling outputs.
SWAP-70 Double Nickase Plasmid (h2) consists of a matched pair of plasmids engineered for high-specificity editing of the SWAP70 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SWAP70. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SWAP70 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SWAP70-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.