SVCT2 CRISPR/Cas9 KO Plasmid (h): sc-401350
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SVCT2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SVCT2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SVCT2 CRISPR/Cas9 KO Plasmid (h) | sc-401350 | 20 µg | $397.00 |
Overview
SLC23A2 encodes the sodium-dependent vitamin C transporter SVCT2, a high-affinity membrane transporter that mediates cellular uptake of ascorbate to maintain intracellular redox balance. By supporting ascorbate-dependent dioxygenases, SVCT2 influences collagen maturation, epigenetic regulation through TET/JmjC enzymes, and hypoxia signaling via prolyl hydroxylases, thereby impacting extracellular matrix homeostasis and transcriptional programs. SVCT2 activity is also linked to mitochondrial function and oxidative stress responses in metabolically active tissues. Altered SLC23A2 expression or ascorbate handling has been associated with susceptibility to oxidative injury and phenotypes relevant to neurobiology, cardiovascular biology, and cancer cell metabolism.
SVCT2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC23A2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC23A2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC23A2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SVCT2 protein expression.
This CRISPR knockout system enables efficient generation of SLC23A2-deficient cell models for investigation of SVCT2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC23A2 exon(s) critical for SVCT2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC23A2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SVCT2 CRISPR/Cas9 KO Plasmid (h) and SVCT2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC23A2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SVCT2 HDR Plasmid (h) and SVCT2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC23A2 homology arms to support homology-directed repair at defined SLC23A2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.