SUSD2 CRISPR/Cas9 KO Plasmid (h): sc-412691
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SUSD2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SUSD2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SUSD2 CRISPR/Cas9 KO Plasmid (h) | sc-412691 | 20 µg | $397.00 |
Overview
SUSD2 (sushi domain containing 2) encodes a single-pass transmembrane protein with extracellular sushi/CCP domains implicated in cell–cell and cell–matrix interactions. Reported functions include modulation of adhesion and migration programs and regulation of immune-relevant signaling at the cell surface, linking SUSD2 to processes such as epithelial organization and stromal–tumor crosstalk. Altered SUSD2 expression has been associated with oncogenic phenotypes in multiple tissues, including changes in invasiveness and metastatic potential. As a surface-associated scaffold, SUSD2 is studied in the context of signaling networks that influence extracellular microenvironment sensing and cellular plasticity.
SUSD2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SUSD2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SUSD2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SUSD2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SUSD2 protein expression.
This CRISPR knockout system enables efficient generation of SUSD2-deficient cell models for investigation of SUSD2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SUSD2 exon(s) critical for SUSD2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SUSD2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SUSD2 CRISPR/Cas9 KO Plasmid (h) and SUSD2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SUSD2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SUSD2 HDR Plasmid (h) and SUSD2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SUSD2 homology arms to support homology-directed repair at defined SUSD2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.