survivin Double Nickase Plasmid (h): sc-400205-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- survivin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- survivin Double Nickase Plasmid (h) and survivin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BIRC5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: survivin Antibody (D-8): sc-17779
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
survivin Double Nickase Plasmid (h) | sc-400205-NIC | 20 µg | $410.00 | |||
survivin Double Nickase Plasmid (h2) | sc-400205-NIC-2 | 20 µg | $410.00 |
BIRC5 encodes survivin, a multifunctional inhibitor of apoptosis family protein that coordinates cell survival with mitotic progression. Survivin is a core component of the chromosomal passenger complex and regulates chromosome alignment, spindle checkpoint fidelity, and cytokinesis, while also modulating caspase-dependent apoptosis and stress responses. Its expression is tightly linked to cell-cycle control and DNA damage signaling, integrating pathways that govern proliferation and programmed cell death. Dysregulated BIRC5 activity is frequently associated with altered apoptosis thresholds and aberrant mitosis, making survivin a widely used molecular node in studies of tumor biology and genome stability.
survivin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BIRC5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BIRC5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BIRC5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BIRC5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.