Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SURF-6 CRISPR/Cas9 KO Plasmid (m): sc-423223
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SURF-6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SURF-6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SURF-6 Antibody (A-5): sc-515439
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SURF-6 CRISPR/Cas9 KO Plasmid (m) | sc-423223 | 20 µg | $397.00 |
Overview
Surf6 encodes SURF-6, a conserved nucleolar protein implicated in ribosome biogenesis and nucleolar homeostasis through roles in pre-rRNA processing and maturation of ribosomal subunits. SURF-6 participates in RNA-binding and nucleolar organization processes that coordinate protein synthesis capacity with cell growth and proliferation programs. Disruption of nucleolar function and ribosome production is tightly linked to cellular stress responses, altered translation, and proliferation defects, making Surf6 relevant for studies of genome stability and growth control in mammalian systems. In mouse models, Surf6 perturbation provides a tractable entry point to investigate how ribosome biogenesis intersects with developmental phenotypes and disease-associated dysregulation of nucleolar pathways.
SURF-6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Surf6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Surf6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Surf6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SURF-6 protein expression.
This CRISPR knockout system enables efficient generation of Surf6-deficient cell models for investigation of SURF-6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Surf6 exon(s) critical for SURF-6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Surf6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SURF-6 CRISPR/Cas9 KO Plasmid (m) and SURF-6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Surf6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SURF-6 HDR Plasmid (m) and SURF-6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Surf6 homology arms to support homology-directed repair at defined Surf6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.