Sur-8 Double Nickase Plasmid (h): sc-409478-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sur-8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Sur-8 Double Nickase Plasmid (h) and Sur-8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SHOC2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sur-8 Antibody (D-8): sc-514779
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sur-8 Double Nickase Plasmid (h) | sc-409478-NIC | 20 µg | $410.00 | |||
Sur-8 Double Nickase Plasmid (h2) | sc-409478-NIC-2 | 20 µg | $410.00 |
SHOC2 encodes Sur-8, a leucine-rich repeat scaffold protein that facilitates RAS-dependent RAF1 activation and amplifies MAPK/ERK signaling downstream of receptor tyrosine kinases. By promoting assembly of RAS–RAF signaling complexes, Sur-8 influences proliferation, differentiation, and feedback regulation within the ERK cascade, with additional connections to EGFR and integrin-linked signaling. Altered SHOC2 function is implicated in developmental syndromes such as Noonan-like disorder with loose anagen hair and has been studied for its contribution to dysregulated MAPK signaling programs relevant to oncogenic RAS contexts. SHOC2 is therefore a useful node for dissecting pathway dynamics, scaffold-dependent signal propagation, and genotype–phenotype relationships in human cells.
Sur-8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SHOC2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SHOC2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SHOC2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SHOC2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.