Sur-8 Antibody (D-8): sc-514779

Sur-8 Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sur-8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Sur-8 (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sur-8, also known as SHOC2 (suppressor of clear homolog (C. elegans)), is a 582 amino acid protein that plays a crucial role in the Ras-MAPK signaling pathway, which is essential for cell growth and differentiation. Sur-8 protein is primarily located in the cytoplasm but translocates to the nucleus upon growth factor stimulation, highlighting a dynamic role in cellular signaling. Sur-8 protein exists in two alternatively spliced isoforms and features 20 leucine-rich repeats (LRR), which are important for protein-protein interactions and signaling modulation. Aberrant N-myristoylation of SHOC2 has been linked to Noonan-like syndrome with loose anagen hair, a genetic disorder characterized by distinctive physical features and hair abnormalities. The gene encoding Sur-8 is located on human chromosome 10q25.2 and murine chromosome 19 D2, making Sur-8 protein a significant target for research in developmental biology and genetic disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Sur-8 Antibody (D-8) References:

1. The leucine-rich repeat protein SUR-8 enhances MAP kinase activation and forms a complex with Ras and Raf.  |  Li, W., et al. 2000. Genes Dev. 14: 895-900. PMID: 10783161
2. Erbin inhibits RAF activation by disrupting the sur-8-Ras-Raf complex.  |  Dai, P., et al. 2006. J Biol Chem. 281: 927-33. PMID: 16301319
3. A phosphatase holoenzyme comprised of Shoc2/Sur8 and the catalytic subunit of PP1 functions as an M-Ras effector to modulate Raf activity.  |  Rodriguez-Viciana, P., et al. 2006. Mol Cell. 22: 217-30. PMID: 16630891
4. Mutation of SHOC2 promotes aberrant protein N-myristoylation and causes Noonan-like syndrome with loose anagen hair.  |  Cordeddu, V., et al. 2009. Nat Genet. 41: 1022-6. PMID: 19684605
5. soc-2 encodes a leucine-rich repeat protein implicated in fibroblast growth factor receptor signaling.  |  Selfors, LM., et al. 1998. Proc Natl Acad Sci U S A. 95: 6903-8. PMID: 9618511
6. SUR-8, a conserved Ras-binding protein with leucine-rich repeats, positively regulates Ras-mediated signaling in C. elegans.  |  Sieburth, DS., et al. 1998. Cell. 94: 119-30. PMID: 9674433

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sur-8 Antibody (D-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Sur-8 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sur-8 Antibody (D-8) Specifications

Alternate Product Name: Sur-8

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 5-304

Epitope Target: Sur-8

Epitope Species: human

Product Reactivity/Detection: Sur-8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Sur-8 Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin