Superoxide Dismutase 2/SOD2 Double Nickase Plasmid (h): sc-400230-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Superoxide Dismutase 2/SOD2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Superoxide Dismutase 2/SOD2 Double Nickase Plasmid (h) and Superoxide Dismutase 2/SOD2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SOD2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Superoxide Dismutase 2/SOD2 Antibody (E-10): sc-137254
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Superoxide Dismutase 2/SOD2 Double Nickase Plasmid (h) | sc-400230-NIC | 20 µg | $410.00 | |||
Superoxide Dismutase 2/SOD2 Double Nickase Plasmid (h2) | sc-400230-NIC-2 | 20 µg | $410.00 |
Human SOD2 encodes mitochondrial manganese superoxide dismutase, a primary enzymatic defense against superoxide radicals generated by oxidative phosphorylation. By converting superoxide to hydrogen peroxide, SOD2 helps maintain mitochondrial redox homeostasis, supports electron transport chain function, and limits oxidative damage to mtDNA, proteins, and lipids. SOD2 activity integrates with antioxidant and stress-response pathways, including NRF2-regulated detoxification and mitochondrial quality control processes such as mitophagy and apoptosis signaling. Dysregulated SOD2 expression or impaired activity is linked to oxidative stress phenotypes observed in cancer metabolism, neurodegeneration, cardiomyopathy, and inflammatory disorders, making it a common target for mechanistic studies of mitochondrial dysfunction.
Superoxide Dismutase 2/SOD2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SOD2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SOD2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SOD2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SOD2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.