Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Superoxide Dismutase 1/SOD1 Double Nickase Plasmid (m): sc-423069-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Superoxide Dismutase 1/SOD1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Superoxide Dismutase 1/SOD1 Double Nickase Plasmid (m) and Superoxide Dismutase 1/SOD1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Sod1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Superoxide Dismutase 1/SOD1 Antibody (B-1): sc-271014
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Superoxide Dismutase 1/SOD1 Double Nickase Plasmid (m) | sc-423069-NIC | 20 µg | $410.00 |
Mouse Sod1 encodes superoxide dismutase 1 (SOD1), a cytosolic Cu/Zn-dependent antioxidant enzyme that catalyzes the dismutation of superoxide radicals to hydrogen peroxide and oxygen, thereby limiting oxidative damage to proteins, lipids, and nucleic acids. SOD1 activity is integrated with cellular redox homeostasis pathways, including glutathione and catalase/peroxiredoxin systems, and influences mitochondrial function, proteostasis, and inflammatory signaling under oxidative stress. Dysregulated SOD1 function or aggregation has been widely studied in the context of neurodegeneration and motor neuron vulnerability, and altered Sod1 expression is also used to model oxidative injury in aging, metabolic stress, and toxicant exposure. As a result, Sod1 is a common target for mechanistic studies of reactive oxygen species (ROS) handling, stress-response signaling, and downstream damage-associated pathways.
Superoxide Dismutase 1/SOD1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Sod1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sod1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sod1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sod1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.