SUMO-1 Antibody (D-11): sc-5308

SUMO-1 Antibody (D-11) is a mouse monoclonal IgG3 kappa light chain antibody that detects SUMO-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SUMO-1 monoclonal antibody (D-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The small ubiquitin-like modifier (SUMO) proteins, which encompass SUMO-1, SUMO-2, and SUMO-3, are integral to numerous cellular processes, particularly in the regulation of protein function through post-translational modifications. SUMO-1 plays a crucial role in cellular signaling pathways, influencing processes such as nuclear transport, transcriptional regulation, and apoptosis. SUMO-1′s ability to modify target proteins, including IkBα, MDM2, p53, PML, and Ran GAP1, underscores its importance in maintaining cellular homeostasis and responding to stress. SUMO-1′s interaction with these proteins can affect their stability and activity, which is vital for proper cellular function and can have implications in various diseases, including cancer and neurodegenerative disorders. The unconjugated forms of SUMO-1, along with SUMO-2 and SUMO-3, are primarily localized to the nuclear membrane and nuclear bodies, highlighting their significance in nuclear processes. Furthermore, while SUMO-2 and SUMO-3 are capable of forming polymeric chains and contribute more extensively to protein modification, SUMO-1′s unique properties make SUMO-1 a key player in the intricate network of cellular regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SUMO-1 Antibody (D-11) References:

1. Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3.  |  Saitoh, H. and Hinchey, J. 2000. J Biol Chem. 275: 6252-8. PMID: 10692421
2. Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9.  |  Tatham, MH., et al. 2001. J Biol Chem. 276: 35368-74. PMID: 11451954
3. SUMO-1 modification of human cytomegalovirus IE1/IE72.  |  Spengler, ML., et al. 2002. J Virol. 76: 2990-6. PMID: 11861864
4. Versatile protein tag, SUMO: its enzymology and biological function.  |  Kim, KI., et al. 2002. J Cell Physiol. 191: 257-68. PMID: 12012321
5. Molecular features of human ubiquitin-like SUMO genes and their encoded proteins.  |  Su, HL. and Li, SS. 2002. Gene. 296: 65-73. PMID: 12383504
6. Ubc9 is essential for viability of higher eukaryotic cells.  |  Hayashi, T., et al. 2002. Exp Cell Res. 280: 212-21. PMID: 12413887
7. Positive and negative regulation of APP amyloidogenesis by sumoylation.  |  Li, Y., et al. 2003. Proc Natl Acad Sci U S A. 100: 259-64. PMID: 12506199
8. The intracellular association of the nucleocapsid protein (NP) of hantaan virus (HTNV) with small ubiquitin-like modifier-1 (SUMO-1) conjugating enzyme 9 (Ubc9).  |  Maeda, A., et al. 2003. Virology. 305: 288-97. PMID: 12573574
9. SUMO-1 modification of the acute promyelocytic leukaemia protein PML: implications for nuclear localisation.  |  Duprez, E., et al. 1999. J Cell Sci. 112 (Pt 3): 381-93. PMID: 9885291

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SUMO-1 Antibody (D-11) has 166 citations in a variety of scientific publications.

Primary antibodies like Anti-SUMO-1 Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SUMO-1 Antibody (D-11) Specifications

Alternate Product Name: SUMO1

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 1-101

Epitope Target: SUMO-1

Epitope Species: human

Product Reactivity/Detection: SUMO-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 166 citations in various scientific publications

Available Conjugates/Forms for SUMO-1 Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin