Sulf-2 CRISPR/Cas9 KO Plasmid (h): sc-403277

Overview

SULF2 encodes Sulf-2, an extracellular endosulfatase that selectively removes 6-O-sulfate groups from heparan sulfate proteoglycans, thereby reshaping the sulfation code that controls ligand availability and receptor engagement. By editing heparan sulfate at the cell surface and in the pericellular matrix, Sulf-2 modulates signaling outputs from pathways such as FGF, WNT, and VEGF, influencing cell proliferation, migration, differentiation, and adhesion. Altered SULF2 expression and heparan sulfate remodeling have been associated with tumor microenvironment regulation, invasive behavior, and angiogenic responses, and are also relevant to fibrotic and inflammatory processes. These functions make SULF2 a useful target for dissecting extracellular matrix–dependent signaling and glycosaminoglycan-mediated regulation of cell communication.

Sulf-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SULF2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SULF2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SULF2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sulf-2 protein expression.

This CRISPR knockout system enables efficient generation of SULF2-deficient cell models for investigation of Sulf-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting SULF2 exon(s) critical for Sulf-2 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple SULF2 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Sulf-2 CRISPR/Cas9 KO Plasmid (h) and Sulf-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SULF2 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Sulf-2 HDR Plasmid (h) and Sulf-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SULF2 homology arms to support homology-directed repair at defined SULF2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.