Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Stomatin Double Nickase Plasmid (h): sc-402491-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Stomatin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Stomatin Double Nickase Plasmid (h) and Stomatin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STOM. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Stomatin Antibody (E-5): sc-376869
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stomatin Double Nickase Plasmid (h) | sc-402491-NIC | 20 µg | $410.00 | |||
Stomatin Double Nickase Plasmid (h2) | sc-402491-NIC-2 | 20 µg | $410.00 |
Human STOM encodes stomatin, a membrane-associated scaffolding protein enriched in lipid rafts that helps organize ion channels and transporters at the plasma membrane. Stomatin contributes to regulation of mechanosensitive and acid-sensing ion channel activity, membrane curvature, and vesicular trafficking, linking it to cellular processes such as osmoregulation and signal transduction. It is highly expressed in erythrocytes where it supports membrane stability and participates in red blood cell homeostasis. Altered stomatin expression or localization has been associated with red cell membrane disorders including hereditary stomatocytosis and with broader changes in membrane excitability relevant to neurological and cancer biology research.
Stomatin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STOM locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STOM. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STOM function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STOM-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.