Stim2 CRISPR/Cas9 KO Plasmid (m): sc-431161
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Stim2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Stim2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stim2 CRISPR/Cas9 KO Plasmid (m) | sc-431161 | 20 µg | $397.00 |
Overview
Stim2 (stromal interaction molecule 2) is an endoplasmic reticulum Ca2+ sensor that couples depletion of ER Ca2+ stores to store-operated calcium entry (SOCE) by regulating Orai channel activity at ER–plasma membrane junctions. In mouse cells, STIM2 helps maintain basal cytosolic Ca2+ levels and tunes the amplitude and duration of Ca2+-dependent signaling that impacts NFAT-driven transcription, synaptic function, and immune cell activation programs. Through these calcium signaling networks, Stim2 is linked to processes including neuronal excitability and plasticity, cytokine responses, and cellular stress adaptation. Dysregulated SOCE and STIM2-dependent signaling have been implicated in disease-relevant mechanisms such as neuroinflammation, autoimmunity, and altered cell survival pathways, making Stim2 a useful target for mechanistic studies.
Stim2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Stim2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Stim2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Stim2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Stim2 protein expression.
This CRISPR knockout system enables efficient generation of Stim2-deficient cell models for investigation of Stim2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Stim2 exon(s) critical for Stim2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Stim2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Stim2 CRISPR/Cas9 KO Plasmid (m) and Stim2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Stim2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Stim2 HDR Plasmid (m) and Stim2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Stim2 homology arms to support homology-directed repair at defined Stim2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.