Stim1 Antibody (D-2): sc-166541
- Stim1 Antibody (D-2) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 441-620 mapping near the C-terminus of Stim1 of human origin
- recommended for detection of Stim1 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG2b BP-HRP is the preferred secondary detection reagent for Stim1 Antibody (D-2) for WB applications. This reagent is now offered in a bundle with Stim1 Antibody (D-2) (see ordering information below).
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Stim1 Antibody (D-2) is a mouse monoclonal IgG2b antibody that detects Stromal Interaction Molecule 1 (Stim1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Stim1 is a crucial cell surface transmembrane glycoprotein that plays a significant role in mediating calcium influx, particularly following the depletion of intracellular calcium stores. Located primarily in the endoplasmic reticulum, Stim1 functions as a calcium sensor due to its EF-hand domain, which undergoes substantial conformational changes in response to fluctuating calcium levels. When intracellular calcium levels drop, Stim1 translocates to the plasma membrane, where Stim1 activates the calcium release-activated calcium (CRAC) channel subunit, TMEM142A/Orai1, facilitating the influx of calcium ions essential for various cellular processes, including secretion and transcription. Additionally, Stim1′s interaction with Stim2 serves as a regulatory mechanism, as Stim2 inhibits Stim1-mediated store-operated calcium entry, highlighting the delicate balance of calcium signaling in cellular functions. The implications of Stim1 extend to tumor growth suppression and the interactions between stromal and hematopoietic cells, making Stim1 a vital protein in both normal physiology and disease states.
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Stim1 Antibody (D-2) References:
- The regulation of STIM1 by phosphorylation. | Pozo-Guisado, E. and Martin-Romero, FJ. 2013. Commun Integr Biol. 6: e26283. PMID: 24505502
- Proteins Interacting with STIM1 and Store-Operated Ca2+ Entry. | Wang, WA. and Demaurex, N. 2021. Prog Mol Subcell Biol. 59: 51-97. PMID: 34050862
- STIM1 is a core trigger of airway smooth muscle remodeling and hyperresponsiveness in asthma. | Johnson, MT., et al. 2022. Proc Natl Acad Sci U S A. 119: PMID: 34949717
- The SOAR of STIM1 interacts with plasma membrane lipids to form ER-PM contact sites. | Cohen, HA., et al. 2023. Cell Rep. 42: 112238. PMID: 36906853
- Role of STIM1 in the Regulation of Cardiac Energy Substrate Preference. | Liu, P., et al. 2023. Int J Mol Sci. 24: PMID: 37685995
- Multifaceted control of T cell differentiation by STIM1. | Gross, S., et al. 2023. Trends Biochem Sci. 48: 1083-1097. PMID: 37696713
- The D84G mutation in STIM1 causes nuclear envelope dysfunction and myopathy in mice. | Bryson, V., et al. 2024. J Clin Invest. 134: PMID: 38300705
- Role of STIM1 in stretch-induced signaling in human airway smooth muscle. | Yao, Y., et al. 2024. Am J Physiol Lung Cell Mol Physiol. 327: L150-L159. PMID: 38771147
- STIM1 mediates methamphetamine-induced neuronal autophagy and apoptosis. | Tian, Q., et al. 2024. Neurotoxicology. 103: 134-145. PMID: 38901802
- Degradation of STIM1 through FAM134B-mediated ER-phagy is potentially involved in cell proliferation. | Kajiho, H. and Sakisaka, T. 2024. J Biol Chem. 300: 107674. PMID: 39128711
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stim1 Antibody (D-2) | sc-166541 | 200 µg/ml | $322.00 | |||
Stim1 Antibody (D-2): m-IgG2b BP-HRP Bundle | sc-548522 | 200 µg Ab; 10 µg BP | $361.00 |