STEAP Antibody (B-4): sc-271872

STEAP Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects STEAP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). STEAP (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The six-transmembrane epithelial antigen of the prostate (STEAP) plays a crucial role in cellular processes, particularly in prostate cancer, where STEAP is overexpressed. STEAP protein is primarily located in the cell membrane and is involved in the transport of ions and small molecules, which is vital for maintaining cellular homeostasis and signaling pathways. STEAP presence in human prostate tissue, along with elevated STEAP levels in various cancer cell lines such as those from the prostate, bladder, colon, ovarian, and Ewing sarcoma, underscores STEAP potential as a biomarker for cancer diagnosis and therapy. Additionally, concentrated STEAP protein levels at cell-cell junctions in the secretory epithelium suggest STEAP involvement in maintaining tissue architecture and function. Mouse STEAP shows 80% homology to human STEAP at both the nucleotide and amino acid levels, and human STEAP gene is located on chromosome 7q21, encoding a 339 amino acid protein.

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STEAP Antibody (B-4) References:

1. STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors.  |  Hubert, RS., et al. 1999. Proc Natl Acad Sci U S A. 96: 14523-8. PMID: 10588738
2. Murine six-transmembrane epithelial antigen of the prostate, prostate stem cell antigen, and prostate-specific membrane antigen: prostate-specific cell-surface antigens highly expressed in prostate cancer of transgenic adenocarcinoma mouse prostate mice.  |  Yang, D., et al. 2001. Cancer Res. 61: 5857-60. PMID: 11479226
3. STEAP, a prostate tumor antigen, is a target of human CD8+ T cells.  |  Alves, PM., et al. 2006. Cancer Immunol Immunother. 55: 1515-23. PMID: 16622681
4. STEAP mRNA detection in serum of patients with solid tumours.  |  Valenti, MT., et al. 2009. Cancer Lett. 273: 122-6. PMID: 18793824
5. The STEAP protein family: versatile oxidoreductases and targets for cancer immunotherapy with overlapping and distinct cellular functions.  |  Grunewald, TG., et al. 2012. Biol Cell. 104: 641-57. PMID: 22804687
6. Six-Transmembrane Epithelial Antigen of the Prostate-1 (STEAP-1)-Targeted Ultrasound Imaging Microbubble Improves Detection of Prostate Cancer In Vivo.  |  Yuan, Y., et al. 2019. J Ultrasound Med. 38: 299-305. PMID: 30027616
7. Utilisation of the STEAP protein family in a diagnostic setting may provide a more comprehensive prognosis of prostate cancer.  |  Burnell, SEA., et al. 2019. PLoS One. 14: e0220456. PMID: 31393902
8. The Tumor Suppressive Roles and Prognostic Values of STEAP Family Members in Breast Cancer.  |  Wu, HT., et al. 2020. Biomed Res Int. 2020: 9578484. PMID: 32802887
9. Comprehensive landscape of STEAP family functions and prognostic prediction value in glioblastoma.  |  Chen, H., et al. 2021. J Cell Physiol. 236: 2988-3000. PMID: 32964440
10. Predictive potential of STEAP family for survival, immune microenvironment and therapy response in glioma.  |  Zhao, Z., et al. 2021. Int Immunopharmacol. 101: 108183. PMID: 34649092
11. Mechanism of stepwise electron transfer in six-transmembrane epithelial antigen of the prostate (STEAP) 1 and 2.  |  Chen, K., et al. 2023. Elife. 12: PMID: 37983176
12. A novel prognostic signature and therapy guidance for hepatocellular carcinoma based on STEAP family.  |  Fu, D., et al. 2024. BMC Med Genomics. 17: 16. PMID: 38191397

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-STEAP Antibody (B-4) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-STEAP Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

STEAP Antibody (B-4) Specifications

Alternate Product Name: STEAP

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-105

Epitope Target: STEAP

Epitope Species: human

Product Reactivity/Detection: STEAP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for STEAP Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin