Stat1 Double Nickase Plasmid (h): sc-400086-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Stat1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Stat1 Double Nickase Plasmid (h) and Stat1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STAT1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Stat1 Antibody (C-136): sc-464
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stat1 Double Nickase Plasmid (h) | sc-400086-NIC | 20 µg | $410.00 | |||
Stat1 Double Nickase Plasmid (h2) | sc-400086-NIC-2 | 20 µg | $410.00 |
STAT1 encodes Stat1, a latent cytoplasmic transcription factor that becomes activated downstream of type I, II, and III interferon receptors through JAK-mediated phosphorylation. Phosphorylated Stat1 forms homodimers or heterodimers (e.g., with STAT2) that translocate to the nucleus to regulate interferon-stimulated genes involved in antiviral defense, antigen presentation, and cell-intrinsic immune surveillance. This signaling axis integrates with inflammatory networks such as NF-κB and can reshape cellular programs including apoptosis, cell-cycle control, and metabolic adaptation. Dysregulated STAT1 activity is associated with altered host–pathogen responses and immune-mediated pathology, and is frequently interrogated in studies of inflammation, infection biology, and tumor–immune interactions.
Stat1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STAT1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STAT1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STAT1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STAT1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.