StAR (h): 293 Lysate: sc-112333

StAR (h): 293 Lysate is derived from the human embryonic kidney 293 cell line, which has been genetically engineered to express the human Steroidogenic Acute Regulatory protein (StAR). StAR is a critical regulator of steroid hormone biosynthesis, facilitating the transport of cholesterol into mitochondria where steroidogenesis begins. The 293 cell line, known for its high transfection efficiency and robust protein production, provides an ideal system for producing StAR lysates. In research, StAR (h): 293 Lysate is utilized to study the molecular mechanisms of steroidogenesis. StAR operates by binding to cholesterol and promoting its transfer across the mitochondrial membrane, a rate-limiting step in the production of steroids. Researchers use this lysate in various biochemical assays, including Western blotting and immunoprecipitation, to analyze StAR expression, post-translational modifications, and interactions with other proteins involved in the steroidogenic pathway. Additionally, StAR (h): 293 Lysate is employed in functional assays to investigate the impact of different regulatory factors on StAR activity and to dissect the signaling pathways that modulate its function. This lysate is instrumental in exploring the effects of StAR mutations and their consequences on cholesterol transport and steroid production. Through these studies, researchers gain insights into the regulatory networks governing steroid biosynthesis and the role of StAR in cellular metabolism and signaling. This research enhances our understanding of the fundamental processes involved in steroidogenesis and cholesterol homeostasis.