Stabilin-1 CRISPR/Cas9 KO Plasmid (h): sc-406773

Overview

STAB1 encodes Stabilin-1, a multifunctional scavenger receptor predominantly expressed by endothelial cells and alternatively activated macrophages, where it supports endocytosis and clearance of diverse ligands including modified lipoproteins and apoptotic cell–derived material. Through its roles in receptor-mediated uptake, intracellular trafficking, and cellular adhesion, Stabilin-1 contributes to vascular homeostasis, immune regulation, and remodeling processes within tissue microenvironments. STAB1 activity is frequently studied in the context of macrophage polarization and stromal–immune crosstalk that shape inflammatory signaling and extracellular matrix turnover. Dysregulated STAB1 expression has been associated with chronic inflammatory states and tumor-associated macrophage biology, providing a mechanistic entry point for examining myeloid contributions to disease-relevant microenvironments.

Stabilin-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the STAB1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the STAB1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the STAB1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Stabilin-1 protein expression.

This CRISPR knockout system enables efficient generation of STAB1-deficient cell models for investigation of Stabilin-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting STAB1 exon(s) critical for Stabilin-1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple STAB1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Stabilin-1 CRISPR/Cas9 KO Plasmid (h) and Stabilin-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the STAB1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Stabilin-1 HDR Plasmid (h) and Stabilin-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by STAB1 homology arms to support homology-directed repair at defined STAB1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.