SSTR1 CRISPR/Cas9 KO Plasmid (h): sc-403410
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SSTR1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SSTR1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SSTR1 Antibody (1F7): sc-293490
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SSTR1 CRISPR/Cas9 KO Plasmid (h) | sc-403410 | 20 µg | $397.00 |
Overview
SSTR1 encodes somatostatin receptor 1, a Gi/o-coupled GPCR that binds somatostatin to dampen adenylyl cyclase activity, reduce intracellular cAMP, and modulate ion channel conductance. Through GPCR signaling, SSTR1 influences MAPK and PI3K-associated cascades and contributes to regulation of neuronal excitability, endocrine secretion, and cell-cycle control in a context-dependent manner. Receptor activity shapes neuroendocrine communication and hormone release pathways, and altered somatostatin signaling has been studied in disorders involving dysregulated secretion, proliferation, and neurotransmission. SSTR1 is therefore a useful target for dissecting inhibitory GPCR networks and pathway cross-talk in human cellular models.
SSTR1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SSTR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SSTR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SSTR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SSTR1 protein expression.
This CRISPR knockout system enables efficient generation of SSTR1-deficient cell models for investigation of SSTR1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SSTR1 exon(s) critical for SSTR1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SSTR1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SSTR1 CRISPR/Cas9 KO Plasmid (h) and SSTR1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SSTR1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SSTR1 HDR Plasmid (h) and SSTR1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SSTR1 homology arms to support homology-directed repair at defined SSTR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.